This ability relies on amplification of sequence fragments from DNA isolated directly from environment (either free DNA-environmental DNA-eDNA or from intact cells in the environment-metagenomic DNA-mDNA), without prior culturing micro-organisms (Steele and Streit 2005).Īlthough analysis of amplicon sequencing results warranted breakthrough in microbial ecology, its careless use may lead to false conclusions. Its popularity stems from the ability to study not only a fraction of culturable micro-organisms (estimated at 0♱–50% (Browne et al. The advent of next-generation sequencing (NGS) in 2005 and associated drop of sequencing costs made it increasingly popular, allowing for assessment of greater number of samples with greater coverage. The technique has a nearly 40-year-long tradition, and initially was performed using Sanger sequencing. Marker gene amplicon sequencing (MGAS) is commonly used in microbial ecology to assess microbial community changes in space and time (Gonzalez et al.
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